TY - JOUR
T1 - In vitro effects of triterpenic acids from olive leaf extracts on the mitochondrial membrane potential of promastigote stage of Leishmania spp
AU - Sifaoui, Ines
AU - López-Arencibia, Atteneri
AU - Martín-Navarro, Carmen Ma
AU - Ticona Huallpara, Juan Carlos
AU - Reyes-Batlle, María
AU - Mejri, Mondher
AU - Jiménez, Antonio Ignacio
AU - Lopez-Bazzocchi, Isabel
AU - Valladares, Basilio
AU - Lorenzo-Morales, Jacob
AU - Abderabba, Manef
AU - Piñero, José E.
N1 - Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
PY - 2014/10/15
Y1 - 2014/10/15
N2 - Protozoan diseases, such as leishmaniasis, are a cause of considerable morbidity throughout the world, affecting millions every year. In this study, two triterpenic acids (maslinic and oleanolic acids) were isolated from Tunisian olive leaf extracts and their in vitro activity against the promastigotes stage of Leishmania (L.) infantum and Leishmania (L.) amazonensis was investigated. Maslinic acid showed the highest activity with an IC50of 9.32 ± 1.654 and 12.460 ± 1.25 μg/ml against L. infantum and L. amazonensis, respectively. The mechanism of action of these drugs was investigated by detecting changes in the phosphatidylserine (PS) exposure, the plasma membrane permeability, the mitochondrial membrane potential and the ATP level production in the treated parasites. By using the fluorescent probe SYTOX® Green, both triterpenic acids showed that they produce a time-dependent plasma membrane permeabilization in the treated Leishmania species. In addition, spectrofluorimeteric data revealed the surface exposure of PS in promastigotes. Both molecules reduced the mitochondrial membrane potential and decreased the ATP levels to 15% in parasites treated with IC90for 24 h. We conclude that the triterpenic acids tested in this study, show potential as future therapeutic alternative against leishmaniasis. Further studies are needed to confirm this.
AB - Protozoan diseases, such as leishmaniasis, are a cause of considerable morbidity throughout the world, affecting millions every year. In this study, two triterpenic acids (maslinic and oleanolic acids) were isolated from Tunisian olive leaf extracts and their in vitro activity against the promastigotes stage of Leishmania (L.) infantum and Leishmania (L.) amazonensis was investigated. Maslinic acid showed the highest activity with an IC50of 9.32 ± 1.654 and 12.460 ± 1.25 μg/ml against L. infantum and L. amazonensis, respectively. The mechanism of action of these drugs was investigated by detecting changes in the phosphatidylserine (PS) exposure, the plasma membrane permeability, the mitochondrial membrane potential and the ATP level production in the treated parasites. By using the fluorescent probe SYTOX® Green, both triterpenic acids showed that they produce a time-dependent plasma membrane permeabilization in the treated Leishmania species. In addition, spectrofluorimeteric data revealed the surface exposure of PS in promastigotes. Both molecules reduced the mitochondrial membrane potential and decreased the ATP levels to 15% in parasites treated with IC90for 24 h. We conclude that the triterpenic acids tested in this study, show potential as future therapeutic alternative against leishmaniasis. Further studies are needed to confirm this.
KW - Bioassay fractionation
KW - Chemotherapy
KW - Leishmania spp
KW - Mechanism of action
KW - Olive leaf extract
UR - http://www.scopus.com/inward/record.url?scp=84907188644&partnerID=8YFLogxK
U2 - 10.1016/j.phymed.2014.08.004
DO - 10.1016/j.phymed.2014.08.004
M3 - Artículo
C2 - 25442278
AN - SCOPUS:84907188644
VL - 21
SP - 1689
EP - 1694
JO - Phytomedicine
JF - Phytomedicine
SN - 0944-7113
IS - 12
ER -