TY - JOUR
T1 - Improved propionic acid production from glycerol
T2 - Combining cyclic batch- and sequential batch fermentations with optimal nutrient composition
AU - Dishisha, Tarek
AU - Ibrahim, Mohammad H.A.
AU - Cavero, Victor Hugo
AU - Alvarez, Maria Teresa
AU - Hatti-Kaul, Rajni
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (ww-1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93mol% and productivity of 0.53gL-1h-1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1gL-1h-1 at initial glycerol up to 120gL-1, and a maximum of 1.63gL-1h-1 from 90gL-1 glycerol.
AB - Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (ww-1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93mol% and productivity of 0.53gL-1h-1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1gL-1h-1 at initial glycerol up to 120gL-1, and a maximum of 1.63gL-1h-1 from 90gL-1 glycerol.
KW - Carbon:nitrogen ratio
KW - Cyclic batch fermentation
KW - Propionibacterium acidipropionici
KW - Propionic acid
KW - Sequential batch fermentation
UR - http://www.scopus.com/inward/record.url?scp=84911417657&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2014.11.013
DO - 10.1016/j.biortech.2014.11.013
M3 - Artículo
C2 - 25460987
AN - SCOPUS:84911417657
VL - 176
SP - 80
EP - 87
JO - Agricultural Wastes
JF - Agricultural Wastes
SN - 0960-8524
ER -