TY - JOUR
T1 - High correlation between Chagas' disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area
AU - Wincker, Patrick
AU - Bosseno, Marie France
AU - Britto, Constança
AU - Yaksic, Nina
AU - Cardoso, Maria Angélica
AU - Morel, Carlos Médicis
AU - Brenière, Simone Frédérique
N1 - Funding Information:
We thank A. Santoro for technical assistance and Catherine Lowndes for correcting the manuscript. This work was supported by grants from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, the INSERM (R6seau Nord-Sud), PAPES-Fioeruz, CNPq and FINEP.
PY - 1994/12/15
Y1 - 1994/12/15
N2 - The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas' disease diagnosis in Bolivia.
AB - The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas' disease diagnosis in Bolivia.
KW - Chagas' disease
KW - Kinetoplast DNA
KW - PCR diagnosis
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=0028173009&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1994.tb07318.x
DO - 10.1111/j.1574-6968.1994.tb07318.x
M3 - Artículo
C2 - 7851750
AN - SCOPUS:0028173009
VL - 124
SP - 419
EP - 423
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 3
ER -