The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas' disease diagnosis in Bolivia.
|Número de páginas||5|
|Publicación||FEMS Microbiology Letters|
|Estado||Publicada - 15 dic. 1994|
Nota bibliográficaFunding Information:
We thank A. Santoro for technical assistance and Catherine Lowndes for correcting the manuscript. This work was supported by grants from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, the INSERM (R6seau Nord-Sud), PAPES-Fioeruz, CNPq and FINEP.