Direct identification of Trypanosoma cruzi natural clones in vectors and mammalian hosts by polymerase chain reaction amplification

S. F. Breniere, M. F. Bosseno, Magda Susana Revollo Zepita Revollo Zepita, M. T. Rivera, Y. Carlier, M. Tibayrenc

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

55 Citas (Scopus)

Resumen

The polymerase chain reaction was used to amplify the highly variable region of the kinetoplast minicircle of Trypanosoma cruzi directly in biological samples (feces of infected Triatomine bugs, blood samples of experimentally infected mice, and artificially infected human blood samples). Hybridization of the amplified DNAs with reference stocks representing different genotypes (natural clones) enabled us to characterize the stocks infecting the biological samples under study. The main interest of this new approach is the diagnosis of T. cruzi infection and simultaneous direct identification of the different natural clones circulating in vectors and mammalian blood without isolation of the stocks. The suitability of this technique for epidemiologic studies is also discussed.

Idioma originalInglés
Páginas (desde-hasta)335-341
Número de páginas7
PublicaciónAmerican Journal of Tropical Medicine and Hygiene
Volumen46
N.º3
DOI
EstadoPublicada - 1992
Publicado de forma externa

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