We describe the isolation and characterization of ten polymorphic microsatellite loci in the Andean pupfish Orestias agassizii. The number of alleles per locus in 25 individuals was high, varying from 5 to 25. Observed and expected mean heterozygosities were 0.750 and 0.767, respectively. The ten loci conformed to the Hardy-Weinberg equilibrium, and we did not detect linkage disequilibrium between loci. Cross-species amplification in O. luteus was entirely successful, and polymorphism was detected in all loci. One locus (A116) showed significant departure from Hardy-Weinberg equilibrium due to homozygote excess. These microsatellites will be useful for assessing the genetic structure and historical demography of Andean pupfishes, which are subject to a global collapse of their stocks.
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Acknowledgments We thank Thierry Oberdorff and Bernard Hugueny for management of the Orestias project. We are thankful to Claudia Zepita, Alexander Flores and Ramón Catari for field assistance. Mark Todd gave useful comments on an early draft of the manuscript. This work was funded by the program ‘‘Action incitative 2008: Diversité des poissons—n°45/Radiation adaptative des Orestias dans les lacs andins’’, from the Département Ressources Vivantes— Institut de Recherche pour le Développement, and Agence Nationale de la Recherche (project ‘‘FISHLOSS’’—ANR-09-PEXT-008-01). This publication was made possible through support provided by the Institut de Recherche pour le Développement—Département Soutien et Formation to YEG.