Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

Ines Sifaoui, Atteneri López-Arencibia, Juan Carlos Ticona Huallpara, Carmen M. Martín-Navarro, María Reyes-Batlle, Mondher Mejri, Jacob Lorenzo-Morales, Antonio Ignacio Jiménez, Basilio Valladares, Isabel Lopez-Bazzocchi, Manef Abderabba, José E. Piñero

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

22 Citas (Scopus)

Resumen

Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.

Idioma originalInglés
Páginas (desde-hasta)S111-S114
PublicaciónExperimental Parasitology
Volumen145
N.ºS
DOI
EstadoPublicada - 1 nov. 2014
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© 2014 Elsevier Inc.

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