Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

Ines Sifaoui; Atteneri López-Arencibia; Juan Carlos Ticona Huallpara; Carmen M. Martín-Navarro; María Reyes-Batlle; Mondher Mejri; Jacob Lorenzo-Morales; Antonio Ignacio Jiménez; Basilio Valladares; Isabel Lopez-Bazzocchi; Manef Abderabba; José E. Piñero

doi:10.1016/j.exppara.2014.02.018

Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

Ines Sifaoui, Atteneri López-Arencibia, Juan Carlos Ticona Huallpara, Carmen M. Martín-Navarro, María Reyes-Batlle, Mondher Mejri, Jacob Lorenzo-Morales, Antonio Ignacio Jiménez, Basilio Valladares, Isabel Lopez-Bazzocchi, Manef Abderabba, José E. Piñero

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

23 Citas (Scopus)

Resumen

Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC₅₀ of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.

Idioma original	Inglés
Páginas (desde-hasta)	S111-S114
Publicación	Experimental Parasitology
Volumen	145
N.º	S
DOI	https://doi.org/10.1016/j.exppara.2014.02.018
Estado	Publicada - 1 nov. 2014
Publicado de forma externa	Sí

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© 2014 Elsevier Inc.

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Sifaoui, I., López-Arencibia, A., Ticona Huallpara, J. C., Martín-Navarro, C. M., Reyes-Batlle, M., Mejri, M., Lorenzo-Morales, J., Jiménez, A. I., Valladares, B., Lopez-Bazzocchi, I., Abderabba, M., & Piñero, J. E. (2014). Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts. Experimental Parasitology, 145(S), S111-S114. https://doi.org/10.1016/j.exppara.2014.02.018

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title = "Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts",

abstract = "Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.",

keywords = "Acanthamoeba, Bioassay fractionation, Chemotherapy, Olive leaf extract",

author = "Ines Sifaoui and Atteneri L{\'o}pez-Arencibia and {Ticona Huallpara}, {Juan Carlos} and Mart{\'i}n-Navarro, {Carmen M.} and Mar{\'i}a Reyes-Batlle and Mondher Mejri and Jacob Lorenzo-Morales and Jim{\'e}nez, {Antonio Ignacio} and Basilio Valladares and Isabel Lopez-Bazzocchi and Manef Abderabba and Pi{\~n}ero, {Jos{\'e} E.}",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",

year = "2014",

month = nov,

day = "1",

doi = "10.1016/j.exppara.2014.02.018",

language = "Ingl{\'e}s",

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Sifaoui, I, López-Arencibia, A, Ticona Huallpara, JC, Martín-Navarro, CM, Reyes-Batlle, M, Mejri, M, Lorenzo-Morales, J, Jiménez, AI, Valladares, B, Lopez-Bazzocchi, I, Abderabba, M & Piñero, JE 2014, 'Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts', Experimental Parasitology, vol. 145, n.º S, pp. S111-S114. https://doi.org/10.1016/j.exppara.2014.02.018

TY - JOUR

T1 - Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

AU - Sifaoui, Ines

AU - López-Arencibia, Atteneri

AU - Ticona Huallpara, Juan Carlos

AU - Martín-Navarro, Carmen M.

AU - Reyes-Batlle, María

AU - Mejri, Mondher

AU - Lorenzo-Morales, Jacob

AU - Jiménez, Antonio Ignacio

AU - Valladares, Basilio

AU - Lopez-Bazzocchi, Isabel

AU - Abderabba, Manef

AU - Piñero, José E.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.

AB - Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.

KW - Acanthamoeba

KW - Bioassay fractionation

KW - Chemotherapy

KW - Olive leaf extract

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U2 - 10.1016/j.exppara.2014.02.018

DO - 10.1016/j.exppara.2014.02.018

M3 - Artículo

C2 - 24726697

AN - SCOPUS:84912094989

SN - 0014-4894

VL - 145

SP - S111-S114

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - S

ER -

Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

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