SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

Cristhian Alvaro Carrasco Villanueva, H. M. Baudel, J. Sendelius, T. Modig, C. Roslander, M. Galbe, B. Hahn-Hägerdal, G. Zacchi, G. Lidén

Research output: Contribution to journalArticlepeer-review

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Abstract

Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting raw material that can be used for fuel ethanol production. In the present study, bagasse was steam pretreated at temperatures between 180 and 205 °C, with holding times of 5-10 min using SO2 as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material for enzymatic hydrolysis. Pretreatment conducted at 190 °C for 5 min gave a pentose yield of 57%, with only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%, 5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment, and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054. The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more than 60%) was achieved by the strain TMB3400 at 2% WIS.

Original languageEnglish
Pages (from-to)64-73
Number of pages10
JournalEnzyme and Microbial Technology
Volume46
Issue number2
DOIs
StatePublished - 5 Feb 2010

Keywords

  • Bioethanol
  • Saccharomyces cerevisiae
  • Sugarcane bagasse
  • Xylose
  • Yeast

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