Phenolics from the Bolivian highlands food plant Ombrophytum subterraneum (Aspl.) B. Hansen (Balanophoraceae): Antioxidant and α-glucosidase inhibitory activity

Nélida Nina, Cristina Theoduloz, Alberto Giménez, Guillermo Schmeda-Hirschmann

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The Balanophoraceae Ombrophytum subterraneum is an endemic highland food plant occurring in Bolivia, northern Argentina and Chile. The upper part of this parasitic plant is eaten fresh. The aim of this work was to characterize the compounds occurring in the edible part and to assess any differences between the inflorescence and tuber. Ethanol extracts of the different plant parts were analyzed for antioxidant activity by the ORAC, TEAC, FRAP, CUPRAC and DPPH methods as well as for the effect on enzymes related to metabolic syndrome (α-amylase, α-glucosidase and pancreatic lipase). The main constituents were isolated by a combination of gel permeation in Sephadex LH-20 and countercurrent chromatography (CCC). Five compounds were isolated and fully identified by spectroscopic and spectrometric means. The new 3′,5,5′,7-tetrahydroxyflavanone 7-O-β-D-1 → 6 diglucoside was isolated for the first time. HPLC-ESI-MS/MS analysis allowed the identification of 19 compounds, including flavanones, flavanols, flavonols, dehydroflavonols and lignans, mainly as glycosides. A strong inhibition towards α-glucosidase was observed for the edible parts (IC50: 1.46 µg/mL) as well as for the tuber and inflorescence (IC50: 1.56 µg/mL and 0.87 µg/mL, respectively). A significant correlation was established between the total phenolic and the antioxidant capacity of the extracts as well as with the content of the new flavanone diglucoside. This is the first comprehensive report on the naturally occurring antioxidants and enzyme inhibitors from this native highland food resource.

Original languageEnglish
Article number109382
JournalFood Research International
Volume137
DOIs
StatePublished - Nov 2020

Bibliographical note

Funding Information:
N.N. thanks to CONICYT for a Doctoral Grant ( Folio 21192237/2019 ). We thank FONDECYT 1170090 for financial support and FONDEQUIP EQM 170023 for the HPLC-MS/MS n measurements. We are indebted to Medardo Nina, Uyuni, Bolivia, for the collection of the plant and the information on the traditional uses and preparations.

Funding Information:
N.N. thanks to CONICYT for a Doctoral Grant (Folio 21192237/2019). We thank FONDECYT 1170090 for financial support and FONDEQUIP EQM 170023 for the HPLC-MS/MSn measurements. We are indebted to Medardo Nina, Uyuni, Bolivia, for the collection of the plant and the information on the traditional uses and preparations.

Publisher Copyright:
© 2020

Keywords

  • Antioxidant activity
  • Bolivia
  • Epicatechin-7-O-glucoside (PubChem CID: 44257085)
  • Flavanone glycosides
  • Highland food plant
  • Ombrophytum subterraneum
  • Parasite plant
  • Quercetin 7-O-glucoside (PubChem CID: 5381351)
  • Taxifolin-7-O-glucoside (PubChem CID: 14282775)
  • α-glucosidase

Fingerprint

Dive into the research topics of 'Phenolics from the Bolivian highlands food plant Ombrophytum subterraneum (Aspl.) B. Hansen (Balanophoraceae): Antioxidant and α-glucosidase inhibitory activity'. Together they form a unique fingerprint.

Cite this