PCR-based diagnosis for Chagas' disease in Bolivian children living in an active transmission area: Comparison with conventional serological and parasitological diagnosis

P. Wincker, J. Telleria, M. F. Bosseno, M. A. Cardoso, P. Marques, N. Yaksic, C. Aznar, P. Liegeard, M. Hontebeyrie, F. Noireau, C. M. Morel, S. F. Breniere

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

A large field study has been performed in the Cochabamba region of Bolivia with the aim of comparing the polymerase chain reaction (PCR) with other diagnostic methods for Chagas' disease. The amplification of Trypanosoma cruzi-specific kinetoplast DNA sequences in blood samples was compared with classical serological methods, specific IgM detection and direct parasite visualization for 268 school children in a single village where Chagas' disease transmission is active. Of 113 children positive by classical serology or buff) coat examination, 106 were detected by PCR (sensitivity: 93.8%). We did not observe any significant difference of PCR sensitivity between initial (IgM and/or buffy coat positive) and indeterminate stage (only IgG positive) patients. Among the remaining 155 children unconfirmed as chagasic (who were either only IgM positive, IgG-, IgM-, and buffy coat-negative) only 1 case was PCR positive. This case may be due to DNA contamination, or to a very recent infection not detected otherwise, or to specific immune depression. These results show that PCR is a very sensitive parasitological test for Chagas' disease in active transmission regions. The future follow-up of the possibly infected patients who were only IgM-positive should clarify the interest of PCR and IgM tests in the detection of starting infections.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalParasitology
Volume114
Issue number4
DOIs
StatePublished - 1997

Keywords

  • Chagas' disease
  • PCR diagnosis
  • Serological diagnosis
  • Trypanosoma cruzi

Fingerprint

Dive into the research topics of 'PCR-based diagnosis for Chagas' disease in Bolivian children living in an active transmission area: Comparison with conventional serological and parasitological diagnosis'. Together they form a unique fingerprint.

Cite this