Metabolic potential of the moderate halophile Yangia sp. ND199 for co-production of polyhydroxyalkanoates and exopolysaccharides

Luis Romero Soto, Habib Thabet, Reuben Maghembe, Denise Gameiro, Doan Van-Thuoc, Tarek Dishisha, Rajni Hatti-Kaul

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7 Scopus citations


Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co-production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB-M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.

Original languageEnglish
Article numbere1160
Issue number1
StatePublished - Jan 2021

Bibliographical note

Funding Information:
The authors are grateful to SIDA (Swedish International Development Cooperation Agency) for supporting the Sweden-Bolivia cooperation project (grant no. 2013/1155) and for the stipend to Luis Romero. Thanks are due to Catalina Fuentes, Daniel Osorio, and Atma-Sol Bustos for help with AF4 theory and experiments.

Publisher Copyright:
© 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.


  • Yangia sp. ND199
  • co-production
  • exopolysaccharide
  • moderate halophile
  • poly(3-hydroxybutyrate)


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