Improved propionic acid production from glycerol: Combining cyclic batch- and sequential batch fermentations with optimal nutrient composition

Tarek Dishisha, Mohammad H.A. Ibrahim, Victor Hugo Cavero, Maria Teresa Alvarez, Rajni Hatti-Kaul

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (ww-1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93mol% and productivity of 0.53gL-1h-1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1gL-1h-1 at initial glycerol up to 120gL-1, and a maximum of 1.63gL-1h-1 from 90gL-1 glycerol.

Original languageEnglish
Pages (from-to)80-87
Number of pages8
JournalBioresource Technology
Volume176
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes

Bibliographical note

Funding Information:
The work was performed within the project BioVinn (Project no. 2008-00849) supported by the Swedish Governmental Agency for Innovation Systems (VINNOVA). Perstorp is acknowledged for coordination of the project. Swedish International Development Cooperation Agency (ASDI/SAREC) is acknowledged for supporting VHC during his stay at Lund University.

Publisher Copyright:
© 2014 Elsevier Ltd.

Keywords

  • Carbon:nitrogen ratio
  • Cyclic batch fermentation
  • Propionibacterium acidipropionici
  • Propionic acid
  • Sequential batch fermentation

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