High correlation between Chagas' disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area

Patrick Wincker, Marie France Bosseno, Constança Britto, Nina Yaksic, Maria Angélica Cardoso, Carlos Médicis Morel, Simone Frédérique Brenière

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas' disease diagnosis in Bolivia.

Original languageEnglish
Pages (from-to)419-423
Number of pages5
JournalFEMS Microbiology Letters
Volume124
Issue number3
DOIs
StatePublished - 15 Dec 1994

Bibliographical note

Funding Information:
We thank A. Santoro for technical assistance and Catherine Lowndes for correcting the manuscript. This work was supported by grants from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, the INSERM (R6seau Nord-Sud), PAPES-Fioeruz, CNPq and FINEP.

Keywords

  • Chagas' disease
  • Kinetoplast DNA
  • PCR diagnosis
  • Trypanosoma cruzi

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