Abstract
The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF-MALS-dRI-UV) for the fractionation and determination of the molar mass, the hydrodynamic radius, and the apparent densities of the aggregates and macromolecules present in wine samples. The results from a set of six Argentinian high-altitude wines showed two main populations: the first population composed of wine colloids with higher UV-specific absorptivity and the second population composed of polysaccharides, such as arabinogalactans. The conformation results showed that population 1 consists of small and dense particles, while population 2 showed high molar masses and lower densities. The results demonstrated the use of AsFlFFF as a new, effective method for the fractionation and characterization of wine colloids and wine macromolecules in red wines with further potential applications.
Original language | English |
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Pages (from-to) | 14564-14576 |
Number of pages | 13 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 68 |
Issue number | 49 |
DOIs | |
State | Published - 9 Dec 2020 |
Bibliographical note
Funding Information:The present study was supported by the Swedish International Development Cooperation Agency (SIDA) in a collaborative project between Universidad Mayor de San Andrés (UMSA, Bolivia) and Lund University (Sweden).
Publisher Copyright:
© 2020 American Chemical Society.
Keywords
- anthocyanin
- AsFlFFF
- polysaccharides
- wine colloids
- wine macromolecules