TY - JOUR
T1 - Evaluation of antileishmanial activity of selected brazilian plants and identification of the active principles
AU - Filho, Valdir Cechinel
AU - Meyre-Silva, Christiane
AU - Niero, Rivaldo
AU - Bolda Mariano, Luisa Nathália
AU - Gomes Do Nascimento, Fabiana
AU - Vicente Farias, Ingrid
AU - Gazoni, Vanessa Fátima
AU - Dos Santos Silva, Bruna
AU - Giménez, Alberto
AU - Gutierrez-Yapu, David
AU - Salamanca, Efrain
AU - Malheiros, Angela
PY - 2013
Y1 - 2013
N2 - This study evaluated extracts, fractions, and isolated compounds from some selected Brazilian medicinal plants against strains of promastigotes of Leishmania amazonensis and L. brasiliensis in vitro. The cell viability was determined, comparing the results with reference standards. The dichloromethane fractions of the roots, stems, and leaves of Allamanda schottii showed IC 50 values between 14.0 and 2.0 g/mL. Plumericin was the main active compound, with IC50 of 0.3 and 0.04 g/mL against the two species of Leishmania analyzed. The hexane extract of Eugenia umbelliflora fruits showed IC50 of 14.3 and 5.7 g/mL against L. amazonensis and L. brasiliensis, respectively. The methanolic extracts of the seeds of Garcinia achachairu and guttiferone A presented IC50 values of 35.9 and 10.4 g/mL, against L. amazonensis, respectively. The ethanolic extracts of the stem barks of Rapanea ferruginea and the isolated compound, myrsinoic acid B, presented activity against L. brasiliensis with IC50 of 24.1 and 6.1 g/mL. Chloroform fraction of Solanum sisymbriifolium exhibited IC50 of 33.8 and 20.5 g/mL, and cilistol A was the main active principle, with IC50 of 6.6 and 3.1 g/mL against L. amazonensis and L. brasiliensis, respectively. It is concluded that the analyzed plants are promising as new and effective antiparasitic agents.
AB - This study evaluated extracts, fractions, and isolated compounds from some selected Brazilian medicinal plants against strains of promastigotes of Leishmania amazonensis and L. brasiliensis in vitro. The cell viability was determined, comparing the results with reference standards. The dichloromethane fractions of the roots, stems, and leaves of Allamanda schottii showed IC 50 values between 14.0 and 2.0 g/mL. Plumericin was the main active compound, with IC50 of 0.3 and 0.04 g/mL against the two species of Leishmania analyzed. The hexane extract of Eugenia umbelliflora fruits showed IC50 of 14.3 and 5.7 g/mL against L. amazonensis and L. brasiliensis, respectively. The methanolic extracts of the seeds of Garcinia achachairu and guttiferone A presented IC50 values of 35.9 and 10.4 g/mL, against L. amazonensis, respectively. The ethanolic extracts of the stem barks of Rapanea ferruginea and the isolated compound, myrsinoic acid B, presented activity against L. brasiliensis with IC50 of 24.1 and 6.1 g/mL. Chloroform fraction of Solanum sisymbriifolium exhibited IC50 of 33.8 and 20.5 g/mL, and cilistol A was the main active principle, with IC50 of 6.6 and 3.1 g/mL against L. amazonensis and L. brasiliensis, respectively. It is concluded that the analyzed plants are promising as new and effective antiparasitic agents.
UR - http://www.scopus.com/inward/record.url?scp=84880170235&partnerID=8YFLogxK
U2 - 10.1155/2013/265025
DO - 10.1155/2013/265025
M3 - Artículo
AN - SCOPUS:84880170235
SN - 1741-427X
VL - 2013
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 265025
ER -