Galipea longiflora (Rutaceae) is a plant able to produce alkaloids of the quinolinic type, which display strong antiparasitic activity against Leishmania strains. Using techniques for in vitro plant cell cultures, a batch culture from this amazonian tree was generated. Different types and concentrations of plant hormones were studied. The combination of 2,4-Dichlorophenoxi acetic acid (5 mg/L) and Kinetine (0.1 mg/L), was very efficient for the induction and production of friable callus from leaves. The callus were used as source of inoculums, for establish cultures in cellular suspension in Murashige & Skoog salt liquid medium and vitamins from the Gamborg's B5 medium, maintained at 25°C on an orbital shaker at 100 rpm, in complete darkness conditions. In the cellular suspension were evaluated the biomass (fresh and dry weight) and the presence of secondary metabolites (alkaloids) in the medium, in order to characterize this suspension culture.
|Translated title of the contribution||Establishment of in vitro cultures of Galipea longiflora, a rutaceae from Bolivian Amazonia|
|Number of pages||5|
|Journal||Latin American Journal of Pharmacy|
|State||Published - Jan 2007|