Enterotoxins, colonization factors, serotypes and antimicrobial resistance of enterotoxigenic Escherichia coli (ETEC) strains isolated from hospitalized children with diarrhea in Bolivia

Claudia Rodas, Rosalía Mamani, Jorge Blanco, Jesus Eulogio Blanco, Gudrun Wiklund, Ann Mari Svennerholm, Åsa Sjöling, Volga Iniguez

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36 Scopus citations

Abstract

Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of bacterial diarrhoea among children in Asia, Africa and Latin America but less investigated in Bolivia. Objective: To determine the relation between enterotoxins, CFs and serotypes as well as the antimicrobial resistance patterns in a set of ETEC isolates collected from hospitalized children with acute diarrhea. In the present study we characterized 43 ETEC strains isolated from 2002 to 2006 from hospitalized children (0-5 years) with acute diarrhea in Bolivia. The strains were analyzed for heat-labile (LT) and heat-stable (ST) enterotoxins and colonization factor (CF) profiles, as well as for serogroups and antimicrobial resistance using phenotypic (ELISA, dot blot, slide agglutination and disc difusion) and genotypic (Multiplex PCR) methods. Among the ETEC isolates tested, 30 were positive for LT, 3 for ST and 10 for LT/ST. Sixty-five percent (28/43) of the strains expressed one or more CF. The most common CFs were CS17 (n = 8) and CFA/I (n = 8). The phenotypical and genotypical results for toxins and CFs were congruent except for CS21 that was amplifed in 10 of the strains by multiplex PCR, but CS21 pili was only detected phenotypically in four of these strains. The ETEC strains had diverse O and H antigens and the most common types were O8:H9 LT CS17 (n = 6; 14%) and O78:HNM LT-ST CFA/I (n = 4; 9%). The analysis of antibiotic resistance showed that 67% (n = 29/43) of the strains were resistant to one or several of the antimicrobial agents tested. Presence of CFs was associated with antibiotic resistance. Conclusion: The most common toxin profile was LT 70%, LT/ST 23% and ST 7%. High antimicrobial resistance to ampicillin among serogroups O6, O8 and O78 were the most common.

Original languageEnglish
Pages (from-to)132-137
Number of pages6
JournalBrazilian Journal of Infectious Diseases
Volume15
Issue number2
DOIs
StatePublished - 2011

Bibliographical note

Funding Information:
This work is part of the UMSA-IBMB “Diarrheal Disease Project” supported by the Swedish Agency for Research Economic Cooperation (SIDA). Support was also obtained from the Swedish Research Council, grant no. 6X-09084, Minis-terio de Ciencia e Innovación (Instituto de Salud Carlos III, Fondo de Investigación Sanitaria, REIPI-RD06/0008-1018 and PS09/01273) and Xunta de Galicia (07MRU036261PR, 09TAL007261PR, 2007/000044-0). Claudia Rodas acknowledges the financial support from the Swedish Institute.

Keywords

  • Bolivia
  • Drug resistance
  • Enterotoxigenic escherichia coli
  • Enterotoxins

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