Two biofilm reactors, using pumice stone and Poraver as biofilm supports, were run, based on the optimization of sulphide production using a factorial design. The maximum H2S concentrations reached were 10 and 15 mm, respectively, both being appropriate for metal precipitation in effluents. The set-up of the pumice stone biofilm reactor is suitable for application in the mining area in the Bolivian Andean region, where this material is widely available. The use of specific primers for sulphate-reducing bacteria groups permits the identification of the sulphide-producing bacteria present in biofilms.
Bibliographical noteFunding Information:
The authors want to thank specially the support of Sida/SAREC in the development of the project Microbial Diversity of Poopo Lake and Desaguadero river basin in La Paz, Bolivia and the financial support from Swedish Institute to support the studies of Tania Pozzo. Britte Marie Pott is specially thanked for giving advice and help with the bioreactors. We are also grateful to Dr. Roberto Romero from Analytical Chemistry, Center for Chemistry and Chemical Engineering – Lund University (Sweden) for his critical assistance in the statistic analysis.
- Pumice stones
- Sulphate-reducing bacteria
- Sulphide production