Bioaerosol sampling optimization for community exposure assessment in cities with poor sanitation: A one health cross-sectional study

Lucas Rocha-Melogno, Olivia Ginn, Emily S. Bailey, Freddy Soria, Marcos Andrade, Michael H. Bergin, Joe Brown, Gregory C. Gray, Marc A. Deshusses

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Evidence of exposure to enteric pathogens through the air and associated risk of infection is scarce in the literature outside of animal- or human-waste handling settings. Cities with poor sanitation are important locations to investigate this aerial exposure pathway as their rapid growth will pose unprecedented challenges in waste management. To address this issue, simple surveillance methods are needed. Therefore, the objectives of this study were to optimize a community exposure bioaerosol surveillance strategy for urban outdoor locations with poor sanitation, and to determine which bioaerosols could contribute to exposure. Passive and active bioaerosol sampling methods were used to characterize the fate and transport of sanitation-related bioaerosols during the rainy and dry seasons in La Paz, Bolivia. Median coliform bacteria fluxes were 71 CFU/(m2 × h) during the rainy season and 64 CFU/(m2 × h) during the dry season, with 38% of the dry season samples testing positive for E. coli. Wind speed, relative humidity and UVB irradiance were identified as significant covariates to consider in bioaerosol transport models in La Paz. Active sampling yielded one positive sample (10%) for human adenovirus (HadV) and one sample (10%) for influenza A virus during the rainy season. HadV was detected at the site with the highest bacterial flux. Four samples (8%) were positive for influenza A virus in the dry season. These findings suggest that aerosols can contribute to community exposure to potentially pathogenic microorganisms in cities with poor sanitation. The use of passive sampling, despite its limitations, can provide quantitative data on microorganisms' viability within realistic timeframes of personal exposure.

Original languageEnglish
Article number139495
JournalScience of the Total Environment
Volume738
DOIs
StatePublished - 10 Oct 2020

Bibliographical note

Funding Information:
This study was supported by the Duke Global Health Institute funding for Global Health Doctoral Scholars and discretionary funding from Marc Deshusses and Gregory Gray at Duke University . We thank Mariana Chambi, Miriam Ajnota and Susan Aguilar for their support in field sampling and Natalie Alarja for her help in sample processing at Duke's One Health laboratory. We acknowledge Carolina Garvizu and Ethan Hicks for their thoughtful insights on transport models and Alyssa Platt, Kim Bourne and Bill Pan for their feedback on our statistical analyses. Lastly, many thanks to Ines Melogno who provided transportation and food during the sampling campaigns and to Nestor Ureña for providing lodging.

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

  • Bioaerosols
  • Exposure
  • Pathogens
  • Sanitation
  • Urban

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