Amaranthus caudatus extract inhibits the invasion of E. coli into uroepithelial cells

Soumitra Mohanty, Silvia Zambrana, Soizic Dieulouard, Witchuda Kamolvit, Vera Nilsén, Eduardo Gonzales, Claes Göran Östenson, Annelie Brauner

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Ethnopharmacological relevance: Amaranthus caudatus is traditionally used to treat infections. Based on its traditional usage, we investigated the effect of A. caudatus on the bladder epithelial cells in the protection of E. coli infection. Materials and Methods: The direct antimicrobial effects of A. caudatus on uropathogenic bacteria were investigated using minimum inhibitory concentration (MIC) assay. Bladder epithelial cell lines T24 and 5637 and uropathogenic E. coli strain #12 were used to investigate the effect of A. caudatus. Bacterial adhesion and invasion into bladder cells treated with A. caudatus was analyzed. Expression of uroplakin-1a (UPK1A), β1 integrin (ITGB1), caveolin-1 (CAV1) and the antimicrobial peptides human β defensin-2 (DEFB4A) and LL-37 (CAMP) was evaluated using RT-PCR. Results: No direct antibacterial effect on E. coli or any of the tested uropathogenic strains was observed by A. caudatus. However, we demonstrated reduced mRNA expression of uroplakin-1a and caveolin-1, but not β1 integrin after treatment of uroepithelial cells, mirrored by the decreased adhesion and invasion of E. coli. A. caudatus treatment did not induce increased gene expression of the antimicrobial peptides, LL-37 and human β-defensin-2. Conclusions: Our results showed that A. caudatus has a protective role on bladder epithelial cells against uropathogenic E. coli infection by decreasing the bacterial adhesion and invasion, thereby preventing infection.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalJournal of Ethnopharmacology
Volume220
DOIs
StatePublished - 28 Jun 2018

Bibliographical note

Funding Information:
This research was funded by the Swedish Research Council ( 56X-20356 ), Stockholm County Council (ALF project) and Swedish Neurological Association (AB) and grants from Karolinska Institutet (AB, SM, WK) and the Swedish International Development Cooperation Agency, SIDA (to SZ, CGÖ and EG). SZ is supported by grants from SIDA. SD is supported by an Amgen Scholarship . WK is supported by grants from Siriraj Hospital Mahidol University, Thailand .

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Adhesion
  • Amaranthus caudatus
  • E. coli
  • Urinary tract infection
  • Uroepithelial cells

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