We detected a new outbreak focus with high incidence of cutaneous leishmaniasis in the Sub Andean region of La Paz. This area was never considered previously as an endemic zone of leishmaniasis. Leishmania stocks from human lesions were isolated: three stocks were explored by pulse field gradient electrophoresis, showing evidence for their affiliation to the L. mexicana complex. Eight stocks were submitted to isoenzyme electrophoresis and compared with five reference strains: L. amazonensis, L. braziliensis, L. chagasi, L. mexicana and L. pifanoi. Close genetic proximity was evidenced between newly isolated parasites and the reference stock of L. amazonensis, whereas high divergence was observed between them and either the L. pifanoi, L. mexicana, L. braziliensis and L. chagasi reference strains. Copyright (C) 1998 Elsevier Science B.V.
Bibliographical noteFunding Information:
We are grateful to Elfride Balanza (IBBA) who provided us with some additional reference strains, as well as to the ORSTOM unit at La Paz for field assistance. This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, grant N° 940902. Karyotyping of parasites was performed with financial support from CEC, contract TS3-CT92-0219.
- Cutaneous leishmaniasis
- L. amazonensis
- Leishmania mexicana complex
- Multilocus enzyme electrophoresis
- Orthogonal field alternated gel electrophoresis